analysis of uv visible spectrophotometer to test the reliability of data
if it is found that the analysis of the test results with the literature values or other instrument analysis of the test results is not consistent, top view instrument conditions (wavelength, spectral bandwidth, scanning speed etc.) are the same, the grade of the instrument (model, stray light, noise, baseline flatness) whether a sample is expired, the operation, whether there is a mistake. some analysis of workers in the adjustment of domestic ultraviolet visible spectrophotometer, test data is often used as compared to the relevant import instrument. however, they do not pay attention to the comparison instrument, instrument conditions, grade of samples etc.. if someone in the acceptance of a domestic ultraviolet visible spectrophotometer, previously used three months once a record imports of imported instruments and grades, test condition and performance and spectrum do. later, and with the imported device under the same conditions to test the samples, the results of testing and three months before the data and spectrum is completely different, and samples have been placed for three months, it has a chemical change takes place, of course, with the three months before the test results may not be the same.
there are a number of pharmaceutical analysis workers or drug workers, they often will make some drugs in the uv visible spectrophotometer instrument data, and the requirement of pharmacopoeia of data compared to the assessment instrument. however, they do not pay attention to according to the requirements of pharmacopoeia for the preparation of test specimens. once found the data does not match, it concluded that the instrument is not good, this is wrong. because sample preparation (sample, measuring tools, solvent) is influence on the results of analysis and test, if not carefully processing, can make the test data generation error. but also consider the conditions of the instrument asked, although the pharmacopoeia has is clear about the request, but did not explain the pharmacopoeia instrument condition, therefore, once found the data with the pharmacopoeia does not comply, repeated testing but also serious changes to the instrument conditions, we can draw a correct conclusion.
some scientific and technical workers in the analysis to the result does not have the testing work, think instrument problems. for example, a sample absorption peak wavelength of 247nm, but the sample is dissolved in acetone, acetone and cut-off wavelength at 330nm, of course, be measured sample absorption wavelength at 247nm, because acetone at 330nm is not transparent. so in addition to the instrument itself should also look for other reasons.
in the daily analysis of test work, people often is proposed to detect the uv visible spectrophotometer, is the use of liquid standard sample or the use of solid standard plate. the author thinks that both have advantages and disadvantages. solid standard chip features, more convenient, easy to carry, and save for a long time, but also can avoid man-made errors generated during preparation of standard sample, but its price is more expensive. characteristics of liquid standard sample is more trouble, preparation of sample, not easy to long-term preservation, operation error and preparation of standard sample when there may lead people, but its price is cheaper.
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